National Institute of Oceanography and Fisheries (NIOF), Egypt
Title: Molecular identification and biochemical constituent of oleaginous marine microalga Nannochloropsis (Eustigmatophyceae), isolated from Mediterranean Sea, Eastern Harbor of Alexandria coast, Egypt
Prof. Dr. Abdel-Wahab Kamel is expert in microalgae mass production at the Marine Invertebrates Lab, Aquaculture Division of the National Institute of Oceanography and Fisheries (NIOF). He has thirty years in experience in economic culture media, low cost technique with reduced production cost of different microalgae species. He mainly concern on reduce of production cost of economical algal species, like Spirulina, Dunaliella and Chlorella. He interested in microalgae isolation, optimum growth conditions, mass production and the utilization of microalgae as live food for invertebrates larval feeding. He has earned his B.Sc. degree in 1974 in Agriculture Science, El-Mansoura University; M.Sc., 1985, in the field of Aquatic Plant, El-Zagazig University, and the Ph.D., 1991, on Phytoplankton, El-Zagazig University. He participated as PI, team leader, in national project under the title (The Development Methods of Mass Production of Natural Food, Phytoplankton), funded from NIOF 2015-2016. He participated as Co-PI in national project under the title The (Biodiesel and Protein Concentrate Production from Marine Microalgae), funded from NIOF 2014-2015. He worked in many national project all dealt with microalgae mass production, low cost techniques. He is a supervisor on many theses in Egypt and published a valuable scientific paper.
Screening of local isolated Egyptian marine micro algal species, with potential for aquaculture, biodiesel and biotechnology, are essential to achieve strong micro algal database with successful commercial applications. In this study, identification of Nannochloropsis, isolated from the eastern south of Mediterranean Sea, was carried out using morphological identification, scanning electron microscope and molecular technique (18S rDNA). Moreover, biochemical constituents (lipid, protein, and carbohydrates) were observed. Our promising isolate was carefully identified as Nannochloropsis in the light of obtaining specific genomic band with 982 bp. Alignment of specific band sequence confirmed our identification as Nannochloropsis oceanica strains LAMB0001, N. oceanica strains CCMP531 and N. oceanica strains IMET1 with 100% of identity. On the other hand, total lipid, protein and carbohydrate of isolated and identified N. oceanica strains LAMB0001 were 37.71±4.27%, 22.81±3.71%, and 18.93±2.19% of dry weight, respectively, which make this strain as a good source for either biodiesel production or aquaculture feedstock.